Skeletochronology, age at maturity and cause of mortality of loggerhead sea turtles Caretta caretta stranded along the beaches of Campania (south-western Italy, western Mediterranean Sea)

Information on demographic and life-history traits of endangered vertebrate species, such as sea turtles, is crucial for planning management and conservation actions. We applied skeletochronology of phalanges to estimate the age of loggerhead turtles, Caretta caretta, found dead stranded along the beaches of Campania (western Mediterranean) from 2013 to 2017. To obtain maturity data, we examined gonads from a subsample of 7 males and 11 females. Overall, curved carapace length (CCL) ranged from 5.6 to 90.8 cm, but for most turtles (89%) it was 50–79.9 cm. Predominance of stranded females (62%) was recorded. Ten out of the eighteen histologically examined gonads allowed estimating maturity. Based on the lines of arrested growth counting, the estimated age of the examined specimens ranged from 0 (hatchling) to 26 years. The modal age was 14 years for males and 17 years for females. The smallest male with spermatogenetic activity had a CCL of 65 cm and was estimated to be 16 years old. The smallest female with follicular development stage, characterising the transition towards adulthood, had a CCL of 69.5 cm and was estimated to be 20 years old. Anthropogenic factors were responsible for 36% of the mortality of individuals, followed by parasitic/infective pathologies (20%).


Introduction
Accurate information on the age at sexual maturity and longevity of individuals is crucial for understanding the structure of a population in its natural environment and implementing appropriate strategies for the management and conservation of threatened species (Avens et al. 2017). This is especially important for species that are still understudied or can be considered model or-In the IUCN Red List of Threatened Species (ver. 2019-1), C. caretta is globally listed as Vulnerable (Casale and Tucker 2017); it is endangered in Italy (Rondinini et al. 2013). Among the most probable causes of the decreasing trend of the Mediterranean populations of this species are the degradation of coastal and open sea habitats, the prolonged illegal trade, and fishery bycatch (Lutcavage et al. 1997;Margaritoulis et al. 2003;Casale et al. 2004;Mingozzi et al. 2007).
Caretta caretta is frequently found dead stranded on the beaches or coastal waters of Italy, including those of Campania (southwestern Italy, Tyrrhenian Sea, western Mediterranean). During 1980During -2008 individuals were recorded along Italian coasts as stranded (2375, of which 77% were dead) or floating (1494, of which approximately 13% were dead) individuals, while the state of the remaining 2069 individuals was not specified .
Between 2012 and 2018, 130 dead or dying individuals of C. caretta were found stranded on Campanian beaches (Istituto Zooprofilattico Sperimentale del Mezzogiorno, Napoli, Italy, unpublished data). However, this number is likely underestimated, since other organisations locally involved in the monitoring of this species do not always freely share their data.
There are several techniques for determining the age of marine turtles, each with some difficulties in application and interpretation. The earliest estimates of age-atsize and age at first reproduction for wild sea turtles were provided using growth data from mark-recapture studies. This remains one of the most common approaches for estimating somatic growth rates, age at sexual maturation, and stage duration in sea turtles (Avens and Snover 2013).
Skeletochronology is one of the most reliable indirect methods for estimating the age and growth rates of marine turtles (for a review see Avens and Snover 2013;Goshe et al. 2016;Avens et al. 2017) and other vertebrates, particularly amphibians (see Guarino et al. 2011Guarino et al. , 2014Sinsch 2015) and reptiles (see Castanet 1994;Guarino et al. 2015). It is a histological method based on the interpretation of the incremental growth marks that are formed in the mineralised tissues of an individual throughout its life cycle, corresponding with the deceleration or stopping of the somatic growth (Castanet and Smirina 1990;Castanet et al. 1993;Andreone and Guarino 2003). For sea turtles, skeletochronological studies have been performed, as a rule, on the humerus, because it has the greatest ratio of cortical to cancellous bone and an evident stratification of its bone matrix, which makes the reading of the growth marks feasible (Zug et al. 1986;Avens and Goshe 2007). However, the phalanges of C. caretta can also be reliably used (Guarino et al. 2004), while the scleral ossicles in the leatherback turtle, Dermochelys coriacea, are more accurate than the humer-us for assessing individual age (Zug and Parham 1996;Avens et al. 2009). In skeletochronological studies, attention is paid to the identification of lines of arrested growth (LAGs) that are formed yearly, trying to discriminate them from other lines or marks that can form occasionally (supplementary marks, sensu Castanet et al. 1993). The annual LAG deposition is guided by endogenous physiological rhythms synchronised with environmental signals (Simmons 1992;Castanet et al. 1993). For sea turtles, annual periodicity of LAG has been demonstrated for Chelonia mydas (Snover et al. 2011) using vital fluorescent labelling and for C. caretta using vital fluorescent labelling (Klinger and Musick 1992;Coles et al. 2001) or known-age samples (Snover and Hohn 2004). Many studies provided validation for carapace length back-calculation from humerus LAG diameter measurements and, therefore, indirectly validated annual LAG deposition (Snover et al. 2007;Goshe et al. 2010;Avens et al. 2012Avens et al. , 2015Avens et al. , 2017. Thus, it is possible to indirectly estimate the age of the individual from the number of LAGs and to obtain information on the individual's growth rates from the spacing between successive LAGs (Castanet and Smirina 1990). However, the number of LAGs visible may be lower than the number actually formed throughout the turtle's life due to bone remodelling that may partially or totally remove some LAGs. On the other hand, by applying the back-calculation method (Castanet et al. 1993;Guarino et al. 2008) or the correction factor equation (Parham and Zug 1997;Petitet et al. 2012;Turner Tomaszewicz et al. 2015), it is possible to estimate the number of totally resorbed LAGs that must be added to the number of visible LAGs to obtain an accurate age estimation.
The aims of this paper are to: (i) explore the body size distribution and age structure of dead stranded loggerhead turtles on the beaches of Campania using phalangeal skeletochronology; (ii) estimate, for the first time, the age at sexual maturity of both male and female C. caretta by matching skeletochronological data to gonad histology; (iii) evaluate the main causes of mortality of the stranded individuals and assess possible relationships between them and particular age/size classes.

Sampling
For age estimation using skeletochronology, we examined 66 phalanges from 65 C. caretta individuals (21 males, 35 females, 9 undetermined sex), dead stranded along the beaches of Campania between March 2013 and May 2017, and 1 from an unsexed individual, held captive from birth until its natural death at the Turtle Rescue Centre of the Zoological Station A, Dohrn, Napoli, Italy (Suppl. material 1: Table S2). The latter individual was held in an outdoor tank during the summer months and inside a greenhouse during the winter months.
To evaluate reproductive status, we analysed the gross morphology and histology of the gonads of 18 of the stranded individuals.
During necropsy (see below), we measured and recorded for each individual its preservation state, curved carapace length from the nuchal notch to the posterior marginal tip (CCL, in cm) (Bolten 1999), sex, and macroscopic examination of the gonad.
Necropsies followed the procedure in Wolke and George (1981), which, briefly, includes: dissection of the dead animal, macroscopic examination of its organs, collection of tissue samples, and taking measurements of the carcass and internal organs. Causes of death were identified, where possible, and classified into three general categories: 1) unknown), when there was no clear evidence of or cause of death; 2) related to human activities, when there was a clearly identifiable anthropogenic cause such as a boat strike, presence of plastic debris and/or of line and hook in the digestive system; 3) related to infectious and/or parasitic diseases.
Next, from each individual we took the right fore flipper and froze it immediately, while small portions of the gonads were fixed in 10% buffered formalin and, after 24 hours, stored in 75% ethanol until laboratory analysis.

Skeletochronology
Skeletochronological analysis was carried out on phalangeal bone sections prepared according to the standard paraffin embedding technique and sectioned using a rotary microtome (Parham and Zug 1997;Guarino et al. 2019). After being thawed, soft tissues were removed from the fore flipper using a scalpel to take the second phalanx of the third finger ( Fig. 1). Before histological preparation, we measured the major and minor axes of each phalanx at the diaphyseal level using a vernier caliper, to the nearest 0.1 mm.
Then the phalanges were fixed in 75% ethanol and a diaphyseal transverse slice of 3-5 mm was removed from each of them at the narrowest point using a manual hacksaw. All the slices were decalcified in 5% nitric acid, following standard protocols (Andreone and Guarino 2003;Guarino et al. 2004;Piovano et al. 2011). The range of decalcification time varied from 5 h (for the hatchling phalanges) to 28 h, depending mainly on the sample size and its porosity. Phalangeal slices were subsequently washed in running tap water for 12 h, dehydrated through a series of graded ethanol baths, cleared with a terpene of natural origin (Bioclear, Bio Optica, Milan, Italy), and embedded with paraffin of 58 °C melting point. Phalangeal cross-sections of 15 µm thick- ness were obtained with a standard rotary microtome (Reichert-Jung/Leica 2045, Germany) and stained with Mayer's Hematoxylin for 30 min. Stained cross-sections were examined with a Leica EZ4 stereomicroscope to check for the best stained sections and to have an entire histological view of the sections (8-20× magnification). We also observed them with a Motic BA340 light microscope equipped with a digital camera at a higher magnification to better distinguish closely packed LAGs near the cortical bone's outer margin. Following standard protocols (Andreone and Guarino 2003;Piovano et al. 2011;Lenz et al. 2016), the counting of LAGs was performed independently by two researchers (FMG and NM or FMG and MM) without prior knowledge of the CCL and sex of the specimens. In the case of discrepancies in LAG count, the sections were read again until consensus was reached. The digital images of the bone sections were optimised, with respect to contrast and intensity, using Adobe Photoshop 6.0 (Adobe Systems Incorporated, San José, California, USA), to enhance the distinctiveness of LAGs. When a LAG appeared to be split into two or more lines, each line was counted as a single LAG. To assess the number of LAGs totally removed by bone remodelling, which progresses centrifugally from the core of the diaphysis, we applied the back-calculation method (Avens and Snover 2013;Avens et al. 2017), using software ImageJ 1.52a (National Institute of Health, USA). In particular, the diameter of all the visible LAGs and that of the external diaphyseal cortical margin were measured in the phalanx sections of the smaller individuals (CCL < 40 cm), where no LAGs were lost, and the measurements thus obtained were compared with the diameters of the innermost, measurable LAGs of the larger individuals. All the variables compared represented the mean ± 2 standard deviations (SDs) of three measurements conducted on three different sections. If the difference between the diameter of the innermost visible LAG of an individual with CCL > 40 cm and the diameter of the external diaphyseal margin of a smaller individual exceeded 2 SD, we inferred that the number of totally lost LAGs in the larger individual was equal to the number observed in the smaller individual. The inferred number of lost LAGs was then added to the number of visible LAGs to estimate the actual age of the largest individuals.
Concerning the validation of the skeletochronological method, our study sample included the phalanges of two dead animals of known age: a hatchling (CCL = 5.6 cm), and an individual raised in captivity for 16 months (CCL = 14.1 cm) (see above and Suppl. material 1: Table S2). Furthermore, we reworked the skeletochronological data of Di Maio et al. (2003) and our unpublished data in order to compare the LAG number detected in the humerus and phalanx of the same individual (n =13).
Among 66 phalanges examined there were also those of a turtle that was tagged and released on 28.09.2013 (Mondello, Palermo) with CCL = 63.4 cm and found dead on 24.03.2014 with CCL = 63.5 cm.

Analysis of age at maturity
Gonads were examined macroscopically; they were then processed using the paraffin embedding technique described above, but without the preliminary decalcification of the tissues. The sections, 7 µm thick, were stained with Mallory Trichrome (Bioptica, Milano) and observed under a Motic BA340 light microscope. Males were considered sexually mature if their testes contained spermatozoa and/or there was evidence of spermatogenetic stages, starting from spermatocytogenesis (Meylan et al. 2002). Females were considered sexually mature if their ovaries contained advanced vitellogenetic follicles and/or residual post-ovulatory follicles according to the criteria in Perez-Bermudez et al. (2012).

Statistical analyses
We used Student's test or the Mann-Whitney non-parametric test to compare two independent groups, depending on whether data were normally distributed or not (Kolmogorov-Smirnov test). Spearman's rank test and linear regression were used to evaluate the relationship between CCL and age. A probability level of p < 0.05 was considered significant.

Body size distribution
The CCL of all examined turtles ranged from 5.6 (hatchling) to 90.8 cm (female adult). Nine turtles were not sexed, owing to the advanced state of decomposition. The CCL mean ± SD was 65.9 ± 8.3 cm in males (n =22) and 66.2 ± 11.3 cm in females (n = 35) (Fig. 2). The CCL means did not differ significantly between the sexes (unpaired t-test, t = 0.09, DF = 55, p = 0.92).

Phalanx histological characteristics and growth marks
Cross-section of the phalanx at the diaphyseal level was formed of an outer cortex and an inner medulla. In specimens with CCL < 30 cm, the cortex was mostly vascularised, whereas the medulla was partially filled with trabecular bone (Fig. 3A, B). In specimens with CCL > 30 cm, the cortex was compact and poorly vascularised, whereas the medulla was completely filled with trabecular bone without the formation of a well-defined medullary cavity (Fig. 3C, D).
Both the cortex and medulla were formed essentially by the deposition of parallel-fibered bone. However, the bone layering was distinct in the cortex (Fig. 3A, C), whereas it was strongly altered in the medulla due to remodelling processes with an enlargement of the blood   vessels and formation of Haversian systems (Fig. 3D). Distinct LAGs were observed in all the phalangeal sections analysed (Fig. 4A, B) except in the hatchling, as expected. The turtle held in captivity for 16 months, with CCL = 14.1 cm, had one LAG, which was close to the outer cortical margin (Fig. 3B). Two wild turtles, with CCLs = 9.8 cm and 16.0 cm, also had only 1 LAG each. Therefore, all these specimens were considered not to have resorbed LAGs during bone growth. The turtle from Palermo stranded with CCL = 63.5 cm had the last LAG confluent with the outer bone margin.

Age estimation
Eleven (85%) out of thirteen specimens used for skeletochronological comparison of different bones showed an identical number of LAGs in the phalanx and humerus (Table 1), while two specimens (15%) differed by only one LAG, indicating that skeletochronology of phalanges is a reliable method for age estimation for C. caretta.
Precise age estimation (number of visible LAGs + number of lost LAGs) was obtained for 59 (90%) out of 66 turtles of the study sample. In two males and five females, age was estimated with an error of 1-6 years due to extensive bone remodelling that did not allow an accurate estimation of the number of lost LAGs. These individuals, including some of the largest specimens (a male with CCL = 80 cm and 25-30 LAGs; a female with CCL = 90 cm and 24-30 LAGs), were excluded from subsequent analyses. Age ranged from 10 to 26 years for males (mean ± SD: 17.35 ± 4.2, n = 20) and 7 to 26 years for females (mean ± SD: 17.1 ± 5.0, n = 30), and did not significantly differ between the sexes (Mann-Whitney U-test, U = 289, p = 0.834). The modal age was 14 years for males and 17 years for females (Fig. 5), but a similar percentage of adult males (25%) and females (26%) were older than 20 years.

Cause of death
Excluding the specimens without a precise age estimation (n = 7), of the 59 turtles necropsied, 21 (10 males, 10 females, 1 unsexed, ~36 %) had their death attributed to anthropogenic causes and 12 (5 males, 4 females, 3 ND, ~20 %) to parasitic/infective pathologies. For 26 specimens (~44%), we could not identify cause of death. Three out of five individuals aged 1-5 years died from parasitic/infective pathologies, while the majority of older specimens died from anthropogenic causes (see Suppl. material 1: Table  S3). Injuries by hook and line were the most common anthropogenic cause of death (n = 13), followed by boat strike (n = 7) and ingestion of plastic debris (n = 1). However, for both sexes, we did not find any relation between age and specific cause of mortality (Suppl. material 1: Table S3).

Age at maturity
About half of the examined gonads were in an advanced autolytic state, with altered tissue organisation due to a poor state preservation, so their gametogenic stage could not be evaluated (Suppl. material 1: Table S4; Fig. 7). In only three females (with CCLs = 69.5 cm, 55.6 cm and 61.3 cm) of the 11 examined, the ovary was in a good preservation state to determine accurately the stage reached in the ovarian cycle (Fig. 7A, B). However, we could also use the ovaries of five females with several follicles only partially affected by the autolytic process and assess the stages of their ovarian cycle. The smallest female with ovaries showing follicular development stage during transition to adulthood had CCL = 69.5 cm and was estimated to be 20 years old. For the males, it was possible to recognise the stage of the spermatogenetic cycle in three out of the seven individuals analysed (Fig.7D). The smallest male with mature gonads had a CCL = 65 cm and was estimated to be 16 years old. The latter, although showing the testicles in autolysis, had seminiferous tubules with large lumen diameters, suggesting the occurrence of active spermatogenesis (Fig. 7E).

Discussion
The body size range (CCL = 5.6-90 cm) of the stranded loggerhead turtles from this study was similar to those reported from around the western Mediterranean Sea (CCL = 7-83 cm in Guarino et al. 2004;13-78.9 cm in Piovano et al. 2011;24-86.5 cm in Casale et al. 2011b). However, the majority of the specimens analysed in this study (~82%) fell between 50 and 79.9 cm CCL. We hypothesise that this body size range characterises the neritic stage of C. caretta in the western Mediterranean Sea and that individuals with CCL < 50 cm or > 80 cm (18% of our sample) can only be found occasionally. However, our study was conducted only on dead stranded turtles, and mark-recapture and length frequency study are necessary to confirm our findings. Interestingly, the sex ratio was clearly female-biased (~1.5:1), for individuals whose sex could be determined. The strong predominance of females has also been reported in stranded green turtles Chelonia mydas (Mascarenhas et al. 2005;Failla et al. 2018), and in a long-term study on live C. caretta (McNeill et al. 2016). Unfortunately, we cannot explain the unbalanced sex ratio of the C. caretta specimens studied by us. In general, species that exhibit environmental sex determination show skewed sex ratios more frequently than species that exhibit genotypic sex determination (Bull 1980). In the Mediterranean, juvenile and adult sex ratios of C. caretta do not differ significantly from 1:1, although estimates of hatchling sex ratios are highly female-biased (Maffucci et al. 2013;Casale et al. 2014). In addition, juvenile sex ratios in the Mediterranean are largely unaffected by immigration of Atlantic individuals into the Mediterranean, as previously suggested (Maffucci et al. 2013). However, it is difficult to understand the possible causes of unbalanced sex ra- tio of species with a complex life cycle, such as sea turtles, by just collecting data on sex (Casale et al. 2006). In fact, a correct interpretation of sex ratios needs additional knowledge on distribution, movement and origin of sea turtles. Therefore, more targeted research is required in response to our findings. However, for species that exhibit temperature-dependent sex determination (TSD), e.g. sea turtles, higher environmental temperatures may lead to single-sex populations and, ultimately, population extinction (Hays et al. 2017). Although for sea turtles these concerns are currently speculative, they may become more relevant under extreme global warming scenarios (Hays et al. 2017).
So far, skeletochronology in sea turtles has used the humerus because it exhibits a lower resorption rate than other skeletal elements, the strong foundation of methodology and the large sample sizes for establishing correction factors to estimate the number of resorbed LAGs and the ability to back-calculate somatic growth rates (Avens and Snover 2013). Although previous papers present the use of ground or polished humerus cross sections for skeletochronological analysis, currently recommended and generally accepted methods involve the preparation of stained sections of decalcified humerus and always present the opportunity to evaluate multiple, sequential sections to find the best one to be used for analysis (Goshe et al. 2009;Avens and Snover 2013). On the other hand, the potential for a reliable skeletochronology of the phalanx, previously suggested by Di Maio et al. (2003), has been confirmed here on 13 individuals of C. caretta where the number and pattern of LAGs observed in the humerus and phalanx of each individual were consistent. In addition, the use of the phalanx instead of the humerus provides practical and technical advantages in skeletochronology because it requires shorter preparation times of decalcified sections. Furthermore, the phalanx, unlike larger bones, would not limit the size of the sample that can fit in the embedding mould and/or in the microtome; it allows the preparation of many serial sections that can be observed and compared, which is essential for a correct and unambiguous age estimation. The main difficulty encountered during this study was the complete loss of LAGs due to bone remodelling. Indeed, the phalanx of C. caretta shows strong resorption within the diaphyseal core but retains the periosteal layering on a wide part of the cortex. Therefore, as for the humerus (Aven and Snover 2013), for most individuals it was possible to estimate the number of lost LAGs from the width of the extant LAGs and for most of the phalangeal sections our results are unambiguous and show that the phalanx can be a useful tool for skeletochronological analysis of C. caretta. Further work aimed at establishing standard phalangeal section measurements and relating those to carapace length, will allow to use the phalanges for somatic growth rate back-calculation.
Different approaches have been used to estimate the age of C. caretta. in the Mediterranean Sea, including capture-mark-recapture (Casale et al. 2009), tail measure-ments (Casale et al. 2005), and skeletochronology (Casale et al. 2011a;Piovano et al. 2011). Our skeletochronological age estimation partially agrees with the results obtained from the skeletochronology of loggerhead turtles stranded in other Mediterranean areas and highlights the variability in the age of C. caretta in the studied marine area. In fact, the age of loggerhead turtles with CCL = 40-80 cm, for which it is possible to make comparisons, ranged from 7-26 years in our study, 7-19 years in Casale et al. (2011a), and 9-30 years in Piovano et al. (2011). Although based on dead stranded animals, our data are in accordance with current knowledge on the general life history patterns of sea turtles in indicating that, excluding the single case of a 7-year-old female, the recruitment of juveniles from oceanic habitat to the neritic habitat in the Mediterranean usually starts after 10 years. In addition, during the neritic stage, the majority of the Mediterranean C. caretta are 10-20 years old, rarely exceed 20 years, and even more rarely reach 30 years. However, it should be noted that different growth rates have been reported for loggerhead turtles of either Mediterranean or Atlantic origin (Piovano et al. 2011). We have not performed genetic analyses to find out the origins of the specimens we have studied, and it cannot be excluded that different origins may have influenced the overall age estimation of our sample.
So far, a variety of methods, including mark and recapture, length frequency and skeletochronology, have been used to estimate the age at maturity of C. caretta and other sea turtle species (Avens and Snover 2013;Avens et al. 2015;Turner Tomaszewicz et al. 2015). However, except for the study by Avens et al. (2015), data on the age at maturity of C. caretta usually concern only the females or combine the sexes (see Avens and Snover 2013), and are based on body size and age at first nesting. The estimates of the age at maturity reported in the literature show a varied picture, ranging from 14.9-29.3 years (CCL = 66.5-87.7 cm) for the Mediterranean, 10-38.9 years (CCL = 86-102.5 cm) for the Atlantic, and 25-36 years (CCL ≤ 91.5 cm) for the Pacific population (Avens and Snover 2013;Turner Tomaszewicz et al. 2015). Our study reports the first data on the age at maturity based on the gonadal histology of both sexes of C. caretta. We have shown that males are potentially able to breed at 16 years, at CCL = 65 cm, whereas females attain sexual maturity at 20 years, at CCL = 69 cm. For females, our estimates of the minimum age at sexual maturity are in accord with those by Piovano et al. (2011) and higher than the results reported by Casale et al. (2011a). Unfortunately, the poor state of preservation of the gonads of stranded turtles with CCL < 69 cm did not allow us to verify whether the average size and age at first maturity could be lower than we have reported. On the other hand, our gonad sample size was small (only 1/6 of all examined turtles provided reliable data to assess maturation state) and further research is needed to confirm our findings. However, to the best of our knowledge, the study by Avens et al. (2015), performed on C. caretta specimens stranded along the Atlantic USA coast, is the only one in which the age of sexual maturity is es-timated separately for males and females using different approaches involving skeletochronological data. In this study, the mean age predictions associated with minimum and mean maturation SCLs (straightline carapace length) were 22.5-25 and 36-38 years for females and 26-28 and 37-42 years for males, respectively. The broad total range of potential age at sexual maturity indicates the absence of a knife-edge maturation threshold for the different populations of C. caretta .
Interestingly, the two main categories of causes of mortality (anthropogenic causes and parasitic/infective pathologies) threaten a range of age and size individuals of C. caretta in Campanian waters. Excluding the individuals ≤ 5 years which prevalently died from parasitic/ infective pathologies (3 out of 5 individuals), a clear correlation between distinct death causes and different age classes was not found. However, considering the sample size for which it was possible to determine the cause of death using necropsy (n = 33), our data strongly emphasise that anthropogenic causes, such as hook and line and boat collisions, represent the more frequent threat (64%) for the loggerhead turtle in southern Italian waters (see Suppl. material 3: Table S3).
In conclusion, using phalangeal skeletochronology, we assessed the age of the loggerhead sea turtles, C. caretta, stranded from 2013 to 2017 on southwestern Italian beaches (western Mediterranean). Furthermore, we also obtained data on the age at maturity by histologically examining gonad samples for about 1/3 of the studied animals (n = 66). Our findings on individual age partially agree with those of previous studies on C. caretta in the Mediterranean Sea and indicate that, at the neritic stage, the ages of most Mediterranean loggerhead turtles range between 10 and 20 years; less frequently it falls between 20 and 30 years. Data from gonadal histology suggest that males attain sexual maturity a little earlier than females (at 16 and 20 years respectively), but in both sexes it is after reaching a CCL > 65 cm.