Mean within-lake pairwise relatedness coefficient rqg across the four Ambystoma populations studied. The green bars are 95% upper and lower expected values for a null distribution generated from 9999 permutations of data from all populations, and enclose the values expected if breeding were panmictic across all populations; relatedness in all sampled populations fell outside the range expected under panmixia. Black bar represents the observed mean relatedness in each population, the black bars are the upper and lower bootstrap value for each population.