A) Map of Mexico showing in light grey the Trans-Mexican Volcanic Belt and in dark grey the State of Mexico. B) Map of the State of Mexico with an elevation raster, the darker areas refer to high elevation. The sampling sites are shown in blue and the polygons represented the natural protected areas: 1. The Corredor Biológico Chichinautzin, 2. Nevado de Toluca Volcano, 3. Zona Protectora Forestal Los Terrenos Constitutivos de las Cuencas de los Ríos Valle Bravo, Malacatepec, Tilostoc y Temascaltepec and 4. Reserva de la Biósfera Santuario Mariposa Monarca. The polygon in yellow represent the distribution of Ambystoma altamirani and in orange the distribution of Ambystoma rivulare according Woolrich-Piña et al. (2017).